The Effect of MicroRNA-143 on the Proliferation and Apoptosis of Leukemia Cell Line U-937 / 中国实验血液学杂志

OBJECTIVE@#To investigate the expression of microRNA-143 (miR-143) in patients with acute myeloid leukemia (AML), and the effect of miR-143 over-expression on the proliferation and apoptosis of AML cells. And to verify the targeting relationship between miR-143 and long non-coding RNA MALAT-1. So as to explore the possible regulatory mechanism of miR-143 in the pathogenesis of AML.@*METHODS@#The expression of miR-143 in bone marrow cells of AML patients was detected by RT-qPCR. After miR-143 was over-expressed in U-937 cell lines, the proliferation of U-937 cell lines was detected by CCK-8, clone formation assay, and flow cytometry. In addition, cell apoptosis was detected by Hoechst 33258 fluorescence staining and flow cytometry. At the same time, bioinformatics, RT-qPCR and dual luciferase reporter gene assay were used to predicted and verified the targeting relationship between miR-143 and MALAT-1.@*RESULTS@#Expression of miR-143 in AML patients was significantly lower than those in normal controls. Over-expressed miR-143 could inhibit the proliferation of U-937 cells and promote the apoptosis of the cell. The miR-143 binding site was located on the MALAT-1 RNA sequence, and MALAT-1 was down-regulated in U-937 cells after over-expressed miR-143. However, the expression of miR-143 showed no significantly changed after MALAT-1 silencing.@*CONCLUSION@#Expression of miR-143 in AML patients is lower than that in normal controls. Over-expression of miR-143 can inhibit the proliferation of U-937 cells and promote its apoptosis. And its mechanism may be related to its targe regulation of MALAT-1.

Effect of anatomic liver resection on prognosis of patients with hepatocellular carcinoma / 第二军医大学学报

Objective To explore the effect of anatomic liver resection (AR) and non-anatomic liver resection (NAR) on the prognosis of patients with hepatocellular carcinoma (HCC). Methods The clinical data of 721 HCC patients, who were treated between July 2008 to July 2009, were collected from Eastern Hepatobiliary Surgery Hospital; the patients included 317 undergoing AR and 404 undergoing NAR. The prognosis of the patients was compared between the two groups by using the Kaplan-Meier method and log-rank test. The influence factors of the prognosis were analyzed by using the Cox proportional hazards regression model. The propensity score matching method was used to eliminate the selection bias in clinical and pathological features. Results The 1-year, 3-year and 5-year overall survival (OS) rates of the 721 patients were 85. 9%, 64. 7% and 51. 5%, respectively; and the 1-year, 3-year and 5-year disease free survival (DFS) rates were 59. 3%, 34. 0% and 25. 5%, respectively. AR conferred better OS than NAR (93. 1%, 74. 5% and 62. 5% vs 80. 2%, 56. 8% and 42. 9%, P< 0. 001, respectively); the same was also true for DFS rates (69. 3%, 41. 3% and 34. 9% vs 51. 4%, 38. 3% and 18. 7%, P< 0. 001, respectively). After propensity matching, AR still achieved better prognosis than NAR (OS rates; 93. 9%, 73. 3%, and 59. 4% vs 86.0%, 62.8%, 52. 8%,P=0. 010; DFS rates; 67.9%, 37.5% and 31. 3% vs 56.8%, 33.1% and 22.6%, P = 0. 024). The results of multivariate analysis showed that the tumor size, tumor number, capsule, liver cirrhosis, microvascular invasion, and method of liver resection were the independent risk factors of OS; and blood transfusion, tumor size, tumor number, capsule, liver cirrhosis, microvascular invasion, and method of liver resection were the independent risk factors of DFS. However, the OS and DFS rates were not significantly different between AR and NAR groups in patients with hepatocirrhosis (P=0. 767 and P=0. 638, respectively). AR group achieved better prognosis than NAR group in the HCC patients without cirrhosis (P<0. 001). Conclusion AR can achieve better prognosis than NAR in HCC patients, but for patients with hepatocirrhosis NAR should be recommended in order to retain better liver function.

Statin Therapy for Primary Prevention of Atrial Fibrillation: Guided by CHADS2/CHA2DS2VASc Score

Atrial fibrillation (AF) is the most common arrhythmia and is associated with increased cardiovascular morbidity and mortality. The anti-arrhythmic effect of statins on AF prevention appears to be highly significant in most clinical studies. However, some discrepancies do exist among different clinical studies. Different clinical settings and types of stains used may explain these differences between trials. The CHADS2 and CHA2DS2VASc scoring systems have been used for stroke risk stratification in AF patients. The recent study suggested that these scores can also be used to guide statin therapy for AF prevention. Patients with higher scores had a higher risk of developing AF and gained more benefits from statins therapy than those with lower scores. This review article focused on the ability of these scores to predict AF prevention by statins.

Power view of transmission of channels and collaterals / 中国针灸

<p><b>OBJECTIVE</b>To describe physiology of channels and circulation of qi and blood from the point of view of mechanics.</p><p><b>METHODS</b>The equation of no compressive normal Newtonian fluid electric osmotic motion are solved by using numerical value method.</p><p><b>RESULTS</b>The results of numerical value calculation show that the flow rate of fluid of channel lines is confirm to the migration velocity of middle-small molecules in literature. When the osmotic coefficient of blood vessel wall and blood pressure of the end of artery changes, the flow rate of qi will correspondingly change, which is confirm with the physiologic reaction at acupuncture. If the static pressure of the channel lines and the inflow condition are changed, it can explain the theory of qi stagnation and blood stasis, and flow of qi inducing blood circulation.</p><p><b>CONCLUSION</b>By using the equation of electric osmotic flow, the theory of traditional Chinese medicine can be explained, which is conducive to understanding the conduction phenomena of electric wave and electric current of channels and collaterals.</p>

Modification of Lejour reduction mammaplasty--mammaplasty of L-shaped scar / 中华整形外科杂志

<p><b>OBJECTIVE</b>To introduce a modification of Lejour reduction mammaplasty.</p><p><b>METHODS</b>With the upper pedical flap as the base of mammaplasty, the lower part of breast was resected while excess skin was pushed to lateral and formed "L"-shaped scar after it was resected.</p><p><b>RESULTS</b>From October 2005 to April 2006, the modified Lejour reduction mammaplasty was applied to 10 mammahypertrophic patients with 20 breasts in sum. The result of operation was good and only "L"-shape scar was left in the lower lateral part of the breast.</p><p><b>CONCLUSIONS</b>This method is easy to perform and could avoid inverted T scar caused by routine mammaplasty technique. This method is worth introducing widely.</p>

Adaptive change of masseter muscle after the ostectomy of mandibular angle in rabbits / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the changes of masseter muscle after reducing the mandibular by measuring some relative index after ostectomy of the mandibular angle along arch line.</p><p><b>METHODS</b>Left mandibular angle ostectomy was performed on New Zealand female rabbits aged three months. The right was not operated as control groups. Five rabbits were examined at the following intervals: 2, 4, 8, 12 and 24 weeks.</p><p><b>RESULTS</b>There were remarkable decreases in the experimented masseter muscle masses and the same type muscle fiber areas compared with the control group (P < 0.05). As for the fiber types, a decrease in type I fibers and an increase in type II fibers were noticed on operative side masseters (P <0.05). Sarcomere lengths in the operative side muscles were significantly reduced and showed statistical significance during first 4 weeks (P < 0.05), but there was no difference between the operative group and the control group after 8 weeks (P > 0.05).</p><p><b>CONCLUSIONS</b>The changes of masseter fibres show reconstruction of masseter muscle after mandibular angle ostectomy.</p>

Relationship between nonsyndromic cleft lip with or without cleft palate (NSCL/P) and genetic polymorphisms of MTHFR C677T and A1298C / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the relationship between nonsyndromic cleft lip with or without cleft palate (NSCL/P) and genetic polymorphism of MTHFR C677T and A1298C in Chinese population.</p><p><b>METHODS</b>Case-control study design was employed. MTHFR genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism techniques (PCR-RFLP).</p><p><b>RESULTS</b>As to MTHFR C677T, 48 heterozygous parents with an affected child were analyzed through case-parental control study using TDT (chi2 = 0. 02, P > 0.05). The genotype frequency and allele frequency of 76 NSCL/P cases and 60 controls were analyzed through case-control study (chi2 = 9.91, P < 0.05). As to MTHFR A1298C, 27 heterozygous parents with an affected child were analyzed through case-parental control study using TDT (chi2 = 4.00, P < 0.05). The genotype frequency and allele frequency of 76 NSCL/P cases and 60 controls were analyzed through case-control study (chi2 = 4.42, P < 0.05).</p><p><b>CONCLUSIONS</b>The genetic polymorphism of MTHFR C677T is associated with the development of NSCL/P, and the genetic polymorphism of MTHFR A1298C is a risk factor for NSCL/P in the Chinese population.</p>